首頁
1
技術資料
2
分光光度計
3
分光光度計原理與結構4
https://www.santorytech.com.tw/custom_62743.html 分光光度計原理與結構 分光光度計原理與結構 Working Principle and StructureDifferent matter has different but special absorbance wavelength point. Also, when at the fixed wavelength point, the absorbance has some relation to the substance’s (Always transparent Solution) concentration and its thickness. The relation can be concluded as the following Formula which is called Lambert-Beer Law. T= I/Io A=KCL= -log I/Io A: Absorbance C: Concentration of the Solution K: Absorbance Coefficient of the Solution L: The length of the Solution in the light path I: The intensity of the light focused on the A/D after it permeate the solution to be measured. Io: The intensity of the light focused on the A/D after it permeate the Solution.

Working Principle and Structure

Different matter has different but special absorbance wavelength point. Also, when at the fixed wavelength point, the absorbance has some relation to the substance’s (Always transparent Solution) concentration and its thickness. The relation can be concluded as the following Formula which is called Lambert-Beer Law.

T= I/Io
A=KCL= -log I/Io
A: Absorbance
C: Concentration of the Solution
K: Absorbance Coefficient of the Solution
L: The length of the Solution in the light path
I: The intensity of the light focused on the A/D after it permeate the solution to be measured.
Io: The intensity of the light focused on the A/D after it permeate the Solution.